dna strand break test
- Guideline or reference source
- ICHS2 Guidance on genotoxicity testing and data interpretation for pharmaceuticals intended for human use
- Definition
- Alkaline treatment that converts certain types of DNA lesions into strand breaks that can be detected by the alkaline elution technique, measuring migration rate through a filter, or by the single cell gel electrophoresis or Comet test (in which cells embedded in a thin layer of gel on a microscope slides are subjected to electric current, causing shorter pieces of DNA to migrate out of the nucleus into a “Comet tail”). The extent of DNA migration is measured visually under the microscope on stained cells.
- Related Term
- dna strand break assay
- dna strand breaks
Send comments to info@qdossier.com Please read disclaimer before using Qsauri.